Olympus BX 51 Fluorescent Microscope
The Olympus BX 51 Fluorescent Microscope is a versatile fluorescent microscope that has transmission, reflection, and metal halide light sources. The microscope also has a Nomarsky filter allowing for differential interference contrast (DIC) microscopy. The MPLFLN-BD lenses with semi-apochromat (corrected for chromatic aberration) are especially designed for darkfield, brightfield, DIC, polarized light, and fluorescence microscopy.
|Olympus BX 51 Fluorescent Microscope|
|Material Processed||No liquids unless contained|
|Supported Processes||Supported Processes|
With both the stage and the nose-piece computerized, stitching in the X-Y plane and Z direction is possible. The filter turret has capacity for six filters that can be easily exchanged. The BX 51 is equipped with a DP72 high sensitivity color camera with a peltier cooled CCD detector allowing for good image capture in low light conditions. Making this microscope perfectly suited for fluorescent work in microfluidic channels.
- Feb 2022 - Updated with more detailed SOP
- Fluorescence Imaging
- Video Image Capture
- Up to 1000x magnification
- Dark field
- Stitching in X, Y and Z
- Transmission and Reflection microscopy
- 5X, 10X, 20X, 50X, 100X objectives
- 10X eye piece
|Mirror cube||Excitation wavelength||Dichoric wavelength||Emission wavelength|
|WUS||330-385 nm||400 nm||420 nm|
|WBS||450-480 nm||500 nm||520 nm|
|WGS||510-550 nm||570 nm||590 nm|
- DIC polarizer
- Light Sources
- Metal halide for fluorescence
- Digital color video camera suitable for fluorescence Olympus DP72, 12.8 megapixel,
- peltier cooled
- pixel-shift technology to attain ultra high resolution
- high sensitivity
- high-speed data transfer.
- Stream Motion 1.7 Software
- CO2 shroud available
- Sample must fit under Objective
- Wafer size up to 6"
- Sample height 1.5" max
- No liquids unless contained
The Olympus BX 51 Fluorescent Microscope is designated as a General class tool. Below is a list of approved materials for the tool. Approved means the material is allowed in the tool under normal circumstances. If a material is not listed, please create a helpdesk ticket or email email@example.com for any material requests or questions.
Materials must be non toxic or corrosive, liquids must be contained.
- This tool does not have specific supported processes. See the SOP on how to use the tool.
- Create a Helpdesk Ticket if you need help imaging your sample, or have specific questions.
Standard operating procedure
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- Read through this page and the Standard Operating Procedure above.
- Accurately complete the checkout quiz. You may retake as necessary until all answers are correct.
- Practice with your mentor or another authorized user until you are comfortable with tool operation.
- If you do not have a mentor who is authorized on the tool open a helpdesk ticket to request training. A tool engineer will contact you to set up a training session.
- Schedule a checkout session with a tool engineer via the helpdesk ticket system. If this checkout is successful, the engineer will authorize you on the tool.