Olympus BX 51 Fluorescent Microscope

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Olympus BX 51 Fluorescent Microscope
Equipment Details
Technology Metrology
Materials Restriction General
Material Processed No liquids unless contained
Equipment Manual
Overview System Overview
Operating Procedure SOP
Supported Processes Supported Processes

The Olympus BX 51 Fluorescent Microscope is a versatile fluorescent microscope that has transmission, reflection, and metal halide light sources. The microscope also has a Nomarsky filter allowing for differential interference contrast (DIC) microscopy. The MPLFLN-BD lenses with semi-apochromat (corrected for chromatic aberration) are especially designed for darkfield, brightfield, DIC, polarized light, and fluorescence microscopy.

With both the stage and the nose-piece computerized, stitching in the X-Y plane and Z direction is possible. The filter turret has capacity for six filters that can be easily exchanged. The BX 51 is equipped with a DP72 high sensitivity color camera with a peltier cooled CCD detector allowing for good image capture in low light conditions. Making this microscope perfectly suited for fluorescent work in microfluidic channels.


  • Feb 2022 - Updated with more detailed SOP


  • Fluorescence Imaging
  • Video Image Capture
  • Up to 1000x magnification
  • Dark field
  • Polarizer
  • Stitching in X, Y and Z
  • Transmission and Reflection microscopy

System overview

Hardware details

  • 5X, 10X, 20X, 50X, 100X objectives
  • 10X eye piece
  • Filters
Mirror cube Excitation wavelength Dichoric wavelength Emission wavelength
WUS 330-385 nm 400 nm 420 nm
WBS 450-480 nm 500 nm 520 nm
WGS 510-550 nm 570 nm 590 nm
  • DIC polarizer
  • Light Sources
    • Halogen
    • Metal halide for fluorescence
  • Digital color video camera suitable for fluorescence Olympus DP72, 12.8 megapixel,
    • peltier cooled
    • pixel-shift technology to attain ultra high resolution
    • high sensitivity
    • high-speed data transfer.
  • Stream Motion 1.7 Software
  • CO2 shroud available

Substrate requirements

  • Sample must fit under Objective
  • Wafer size up to 6"
  • Sample height 1.5" max
  • No liquids unless contained

Material restrictions

The Olympus BX 51 Fluorescent Microscope is designated as a General class tool. Below is a list of approved materials for the tool. Approved means the material is allowed in the tool under normal circumstances. If a material is not listed, please create a helpdesk ticket or email info@lnf.umich.edu for any material requests or questions.

Materials must be non toxic or corrosive, liquids must be contained.

Supported processes

  • This tool does not have specific supported processes. See the SOP on how to use the tool.
  • Create a Helpdesk Ticket if you need help imaging your sample, or have specific questions.

Standard operating procedure

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Checkout procedure

  1. Read through this page and the Standard Operating Procedure above.
  2. Accurately complete the checkout quiz. You may retake as necessary until all answers are correct.
  3. Practice with your mentor or another authorized user until you are comfortable with tool operation.
  4. If you do not have a mentor who is authorized on the tool open a helpdesk ticket to request training. A tool engineer will contact you to set up a training session.
  5. Schedule a checkout session with a tool engineer via the helpdesk ticket system. If this checkout is successful, the engineer will authorize you on the tool.