Olympus BX 51 Fluorescent Microscope
| Olympus BX 51 Fluorescent Microscope | |
|---|---|
 | |
| Equipment Details | |
| Technology | Metrology |
| Materials Restriction | General |
| Material Processed | No liquids unless contained |
| Equipment Manual | |
| Overview | System Overview |
| Operating Procedure | SOP |
| Supported Processes | Supported Processes |
The Olympus BX 51 Fluorescent Microscope is a versatile fluorescent microscope that has transmission, reflection, and metal halide light sources. The microscope also has a Nomarsky filter allowing for differential interference contrast (DIC) microscopy. The MPLFLN-BD lenses with semi-apochromat (corrected for chromatic aberration) are especially designed for darkfield, brightfield, DIC, polarized light, and fluorescence microscopy.
With both the stage and the nose-piece computerized, stitching in the X-Y plane and Z direction is possible. The filter turret has capacity for six filters that can be easily exchanged. The BX 51 is equipped with a DP72 high sensitivity color camera with a peltier cooled CCD detector allowing for good image capture in low light conditions. Making this microscope perfectly suited for fluorescent work in microfluidic channels.
Contents
Announcements
- Feb 2022 - Updated with more detailed SOP
Capabilities
- Fluorescence Imaging
- Video Image Capture
- Up to 1000x magnification
- Dark field
- Polarizer
- Stitching in X, Y and Z
- Transmission and Reflection microscopy
System overview
Hardware details
- 5X, 10X, 20X, 50X, 100X objectives
- 10X eye piece
- Filters
| Mirror cube | Excitation wavelength | Dichoric wavelength | Emission wavelength |
|---|---|---|---|
| WUS | 330-385 nm | 400 nm | 420 nm |
| WBS | 450-480 nm | 500 nm | 520 nm |
| WGS | 510-550 nm | 570 nm | 590 nm |
- DIC polarizer
- Light Sources
- Halogen
- Metal halide for fluorescence
- Digital color video camera suitable for fluorescence Olympus DP72, 12.8 megapixel,
- peltier cooled
- pixel-shift technology to attain ultra high resolution
- high sensitivity
- high-speed data transfer.
- Stream Motion 1.7 Software
- CO2 shroud available
Substrate requirements
- Sample must fit under Objective
- Wafer size up to 6"
- Sample height 1.5" max
- No liquids unless contained
Material restrictions
The Olympus BX 51 Fluorescent Microscope is designated as a General class tool. Below is a list of approved materials for the tool. Approved means the material is allowed in the tool under normal circumstances. If a material is not listed, please create a helpdesk ticket or email info@lnf.umich.edu for any material requests or questions.
Materials must be non toxic or corrosive, liquids must be contained.
Supported processes
- This tool does not have specific supported processes. See the SOP on how to use the tool.
- Create a Helpdesk Ticket if you need help imaging your sample, or have specific questions.
Standard operating procedure
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Checkout procedure
- Read through this page and the Standard Operating Procedure above.
- Accurately complete the checkout quiz. You may retake as necessary until all answers are correct.
- Practice with your mentor or another authorized user until you are comfortable with tool operation.
- If you do not have a mentor who is authorized on the tool open a helpdesk ticket to request training. A tool engineer will contact you to set up a training session.
- Schedule a checkout session with a tool engineer via the helpdesk ticket system. If this checkout is successful, the engineer will authorize you on the tool.
