Difference between revisions of "Optical microscopy"

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In order to image and measure objects in the micron range it is necessary the use of a microscope. Microscope technology has advanced tremendously with the
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Set the technology or technology group that this is a member of
 
Set the technology or technology group that this is a member of
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--> {{Infobox technology
 
|image =020.JPG|
 
|image =020.JPG|
|technology = {{#var:parent|Optical Microscopy}}
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|technology = {{#var:parent|Technology}}
|names = {{#if: {{#var:acronym}} | {{#var:acronym}},&nbsp; |}} Epi-fluorescense
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|names = Epi-fluorescence
 
|equipment = Olympus BX51, Nikon Eclipse LV150, Nikon MM-40, Nikon AZ100 Multi zoom,  
 
|equipment = Olympus BX51, Nikon Eclipse LV150, Nikon MM-40, Nikon AZ100 Multi zoom,  
 
|materials = wafers, devices, cells, materials,  
 
|materials = wafers, devices, cells, materials,  
 
}}
 
}}
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[[{{PAGENAME}}]]
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In order to image and measure objects in the micron range it is necessary the use of a microscope. Microscope technology has advanced tremendously with the
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==Equipment==
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[[Olympus BX 51 Fluorescent Microscope]]<br />
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[[Olympus IR Microscope]]<br />
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[[Nikon Eclipse LV150]]<br />
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[[Nikon MM-40]]<br />
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[[Nikon AZ100 Multi Zoom]]
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==Technologies==
 
==Technologies==
 
===Optical Microscopy===
 
===Optical Microscopy===
  
The details of optical microscopy can be found in the well known work by Davidson and Abramowitz<ref>https://micro.magnet.fsu.edu/primer/pdfs/microscopy.pdf</ref> as well as in Olympus<ref>http://olympus.magnet.fsu.edu/</ref> and Nikon Microscopy U<ref>http://www.microscopyu.com/tutorials/</ref>.
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The details of optical microscopy can be found in the well known work by Davidson and Abramowitz<ref>https://micro.magnet.fsu.edu/primer/pdfs/microscopy.pdf</ref><ref>http://ac.els-cdn.com/S0091679X06810010/1-s2.0-S0091679X06810010-main.pdf?_tid=645621dc-d990-11e5-8b97-00000aacb35e&acdnat=1456165168_cf87db97d04aaf4f8cc6ca3efbceafab</ref> as well as in Olympus<ref>http://olympus.magnet.fsu.edu/</ref> and Nikon Microscopy U<ref>http://www.microscopyu.com/tutorials/</ref>.
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The basics parts of a Microscope are illustrated in the figure below:<br />
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[[File:Basics.jpg|frame|center|basic parts of a Microscope<ref>http://www.microscopyu.com/tutorials</ref>|link=]]<br />
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These days microscopes are equipped with more and more advanced CCD digital cameras, which has revolutionized the image caption, interpretation and manipulation. Also, improved designed objective lenses, new light sources, and motorized stages  have taken digital microscopy to levels of image quality not found even 10 years ago. Still, microscope optics physics is the same, so the same basic elements have to be considered when choosing the right microscopy for the task at hand<ref>http://ac.els-cdn.com/B0123694019005799/3-s2.0-B0123694019005799-main.pdf?_tid=08496042-d991-11e5-9d1b-00000aacb362&acdnat=1456165443_eb4672f672413c71c55c3ccdb217abe3</ref>
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* Light Source
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* Condenser
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* Objective
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* Filters
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* Eye pieces
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* Stage
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* Stand
  
 
===Fluorescent Microscopy===
 
===Fluorescent Microscopy===
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==Figures of Merit==
 
==Figures of Merit==
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* Resolution
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* Depth of Field
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* Image mode
 
* Magnification
 
* Magnification
 
* Sharpness
 
* Sharpness
 
* Numerical Aperture
 
* Numerical Aperture
 
* Contrast
 
* Contrast
* light source
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* Light source
  
 
==Applications==
 
==Applications==
How is this technology used in nanofabrication and what types of devices/research areas is it useful in?
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Cell biology, microfabrication, geology, crystallography, etc.
 
 
==Equipment==
 
<!-- This section may be dropped if the group is very broad -->
 
Specific equipment for each technology can be found on its page above. Additionally, below is a list of all metrology equipment in the LNF:
 
<categorytree mode=pages>{{PAGENAME}} equipment</categorytree>
 
[[Olympus BX 51 Fluorescent Microscope]]<br />
 
[[Olympus IR Microscope]]
 
  
 
==See also==
 
==See also==
Other related wiki pages
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[[https://drive.google.com/drive/u/2/folders/0ByIMBpj2dup-MzNWNHA2RlVMbTg]]
  
 
==References==
 
==References==
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==Further reading==
 
==Further reading==
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*[http://lnf-wiki.eecs.umich.edu/wiki/User_Resources#LNF_Tech_Talks_.28technology_seminar_series.29 LNF Tech Talk for Metrology]
 
* Other stuff, e.g. technology workshop slides
 
* Other stuff, e.g. technology workshop slides
 
* External links (can be in another section below, if appropriate)
 
* External links (can be in another section below, if appropriate)
  
 
<!-- Do not add anything below this point besides categories. -->
 
<!-- Do not add anything below this point besides categories. -->
[[Category:{{PAGENAME}}| ]]
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[[Category:Metrology]]
[[Category:Technology]]
 

Latest revision as of 09:13, 31 March 2020


Optical microscopy
020.JPG
Technology Details
Other Names Epi-fluorescence
Technology Metrology
Equipment Olympus BX51, Nikon Eclipse LV150, Nikon MM-40, Nikon AZ100 Multi zoom,
Materials wafers, devices, cells, materials,

Optical microscopy

In order to image and measure objects in the micron range it is necessary the use of a microscope. Microscope technology has advanced tremendously with the

Equipment

Olympus BX 51 Fluorescent Microscope
Olympus IR Microscope
Nikon Eclipse LV150
Nikon MM-40
Nikon AZ100 Multi Zoom

Technologies

Optical Microscopy

The details of optical microscopy can be found in the well known work by Davidson and Abramowitz[1][2] as well as in Olympus[3] and Nikon Microscopy U[4].

The basics parts of a Microscope are illustrated in the figure below:

basic parts of a Microscope[5]


These days microscopes are equipped with more and more advanced CCD digital cameras, which has revolutionized the image caption, interpretation and manipulation. Also, improved designed objective lenses, new light sources, and motorized stages have taken digital microscopy to levels of image quality not found even 10 years ago. Still, microscope optics physics is the same, so the same basic elements have to be considered when choosing the right microscopy for the task at hand[6]

  • Light Source
  • Condenser
  • Objective
  • Filters
  • Eye pieces
  • Stage
  • Stand

Fluorescent Microscopy

Describe the technology, particularly including why you might use it over another within the same group.

Infrared Microscopy

Main article: Infrared microscopy

Here's another technology in that group.

Figures of Merit

  • Resolution
  • Depth of Field
  • Image mode
  • Magnification
  • Sharpness
  • Numerical Aperture
  • Contrast
  • Light source

Applications

Cell biology, microfabrication, geology, crystallography, etc.

See also

[[1]]

References

Further reading

  • LNF Tech Talk for Metrology
  • Other stuff, e.g. technology workshop slides
  • External links (can be in another section below, if appropriate)