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Optical microscopy

Optical microscopy
Technology Details
Other Names Epi-fluorescence
Technology Metrology
Equipment Olympus BX51, Nikon Eclipse LV150, Nikon MM-40, Nikon AZ100 Multi zoom,
Materials wafers, devices, cells, materials,

In order to image and measure objects in the micron range it is necessary the use of a microscope. Microscope technology has advanced tremendously with the




Optical Microscopy

The details of optical microscopy can be found in the well known work by Davidson and Abramowitz[1][2] as well as in Olympus[3] and Nikon Microscopy U[4].

The basics parts of a Microscope are illustrated in the figure below:

basic parts of a Microscope[5]

These days microscopes are equipped with more and more advanced CCD digital cameras, which has revolutionized the image caption, interpretation and manipulation. Also, improved designed objective lenses, new light sources, and motorized stages have taken digital microscopy to levels of image quality not found even 10 years ago. Still, microscope optics physics is the same, so the same basic elements have to be considered when choosing the right microscopy for the task at hand[6]

  • Light Source
  • Condenser
  • Objective
  • Filters
  • Eye pieces
  • Stage
  • Stand

Fluorescent Microscopy

Describe the technology, particularly including why you might use it over another within the same group.

Infrared Microscopy

Main article: Infrared microscopy

Here's another technology in that group.

Figures of Merit

  • Resolution
  • Depth of Field
  • Image mode
  • Magnification
  • Sharpness
  • Numerical Aperture
  • Contrast
  • Light source


Cell biology, microfabrication, geology, crystallography, etc.

See also



Further reading

  • LNF Tech Talk for Metrology
  • Other stuff, e.g. technology workshop slides
  • External links (can be in another section below, if appropriate)